Molecular characterization and Antibiotic resistance profiles of Escherichia coli extended-spectrum β-lactamases producer strains isolated from urine samples in Benin

  • Wassiyath Mousse Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculté des Sciences et Techniques/Université d’Abomey-Calavi, Cotonou, Benin
  • Haziz Sina Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculté des Sciences et Techniques/Université d’Abomey-Calavi, Cotonou, Benin
  • Mamadou Wele Université des Sciences et Techniques de Bamako (USTTB), Bamako, Mali
  • Nicodeme Chabi Ecole Polytechnique d’Abomey-Calavi, (EPAC) – Benin
  • Durand Dah Nouvlessounon Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculté des Sciences et Techniques/Université d’Abomey-Calavi, Cotonou, Benin
  • Farid T. Bade Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculté des Sciences et Techniques/Université d’Abomey-Calavi, Cotonou, Benin
  • Faridath Chabi Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculté des Sciences et Techniques/Université d’Abomey-Calavi, Cotonou, Benin
  • Severin Anagonou Faculté des Sciences et Santé, Université d’Abomey-Calavi. ISBA, Champ de foire, Cotonou, Benin
  • Adolphe Adjanohoun Centre de Recherches Agricoles Sud/ Institut National des Recherches Agricoles du Bénin. Niaouli, Benin
  • Lamine Baba-Moussa Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculté des Sciences et Techniques/Université d’Abomey-Calavi, Cotonou, Benin

Abstract

Urinary tract infections are the second common reason of medical consultations and antibiotics prescription. Escherichia coli is known to cause most urinary tract infections. The aim of this study was to characterize and determine the antibiotic resistance profile of E. coli extended-spectrum βlactamases (ESBL) producer strains isolated from urine samples. The urine samples collected came from hospitalized and non-hospitalized patient referred to Hubert Koutoukou Manga (HKM), National and University Hospital Center (Cotonou, Benin). The resistance to antibiotics was determined according to the disk diffusion method. The production of penicillinase and ESBLs was researched respectively by the acidimetric test and double disk synergy method. The presences of genes encoding βlactamases were detected by Polymerase Chain Reaction (PCR). Our data revealed that 60 % of E. coli strains (101) were isolated from female patients. Also, 69.31 % of the strains were isolated from non-hospitalized patients. The high resistance levels were recorded with amoxicillin (96.04 %) and amoxicillin + clavulanic acid (66.34 %). Twenty percent (20%) of strains were ESBLs. Among ESBLs strains, 70% comes from non-hospitalized patients. Eighty percent of E. coli strains produced penicillinase among which 25 % were ESBL producers. All the ESBL producers strains carried blaTEM gene whereas only 30 % carried the blaSHV gene. This study updates the data on the prevalence to antibiotic resistance of E. coli ESBL producers strains for better management of urinary tract infections.

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Published
2018-10-31
How to Cite
Mousse, W., Sina, H., Wele, M., Chabi, N., Nouvlessounon, D. D., Bade, F. T., Chabi, F., Anagonou, S., Adjanohoun, A., & Baba-Moussa, L. (2018). Molecular characterization and Antibiotic resistance profiles of Escherichia coli extended-spectrum β-lactamases producer strains isolated from urine samples in Benin. European Scientific Journal, ESJ, 14(30), 323. https://doi.org/10.19044/esj.2018.v14n30p323